Synthesizing double haploid hexaploid wheat populations based on a spontaneous alloploidization process.
نویسندگان
چکیده
Doubled haploid (DH) populations are useful to scientists and breeders in both crop improvement and basic research. Current methods of producing DHs usually need in vitro culture for extracting haploids and chemical treatment for chromosome doubling. This report describes a simple method for synthesizing DHs (SynDH) especially for allopolyploid species by utilizing meiotic restitution genes. The method involves three steps: hybridization to induce recombination, interspecific hybridization to extract haploids, and spontaneous chromosome doubling by selfing the interspecific F(1)s. DHs produced in this way contain recombinant chromosomes in the genome(s) of interest in a homogeneous background. No special equipment or treatments are involved in the DH production and it can be easily applied in any breeding and/or genetic program. Triticum turgidum L. and Aegilops tauschii Coss, the two ancestral species of common wheat (Triticum aestivum L.) and molecular markers were used to demonstrate the SynDH method.
منابع مشابه
-
One of the ways of haploid production is a cross between wheat and maize. Recently success has been achieved in this method, leading to the production of zygotes; these zygotes generally undergo elimination of maize chromosomes and thus lead to production of wheat haploid plant. The purpose of this study was to develop an efficient method of haploid production via maize pollination in short dur...
متن کاملThe genetic study utility of a hexaploid wheat DH population with non-recombinant A- and B-genomes
To study the D-genome of the wild wheat relative Aegilops tauschii Cosson at the hexaploid level, we developed a synthetic doubled-haploid (DH) hexaploid wheat population, SynDH3. This population was derived from the spontaneous chromosome doubling of triploid F1 hybrid plants obtained from a cross between Triticum turgidum ssp. dicoccon PI377655 and A. tauschii ssp. strangulata AS66 × ssp. tau...
متن کاملبررسی روابط فیلوژنتیکی ژنهای واکسی در گندمهای وحشی و زراعی با استفاده از PCR چندگانه
Waxy proteins are responsible for amylase synthesis in wheat seeds, being encoded by three waxy genes (Wx-A1, Wx-B1 and Wx-D1) in hexaploid wheat which have an important role in starch quality. The purpose of this study was to investigate phylogenetic relationship between waxy genes in wild and cultivated wheat using Multiplex-PCR. To this end, 71 populations from 8 Aegilops and 4 wheat wild sp...
متن کاملMapping‐by‐sequencing in complex polyploid genomes using genic sequence capture: a case study to map yellow rust resistance in hexaploid wheat
Previously we extended the utility of mapping-by-sequencing by combining it with sequence capture and mapping sequence data to pseudo-chromosomes that were organized using wheat-Brachypodium synteny. This, with a bespoke haplotyping algorithm, enabled us to map the flowering time locus in the diploid wheat Triticum monococcum L. identifying a set of deleted genes (Gardiner et al., 2014). Here, ...
متن کاملHeritability of Grain Yield and Its Components and Selection for Drought Tolerance in Wheat Double Haploid Lines
Drought is one of the most important environmental factors affecting plant growth. The double haploid method is one of the most common breeding methods in wheat. Using old cultivars in cross-breeding programs can be effective for restoring genes lost during evolution. In this study, in order to evaluate the genetic diversity and to select the drought-tolerant introgression wheat lines, 151 doub...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of genetics and genomics = Yi chuan xue bao
دوره 38 2 شماره
صفحات -
تاریخ انتشار 2011